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The remaining brain sections and systemic organs were fixed in 10% buffered formalin for 48 hours

The spectra from fractions F1F3, where proteins elute, were the same as the blank (Fig

Fusion constructs ROGFP2 WT -PFGRX WT , ROGFP2 WT -PFGRX C88S , and ROGFP2 WT -PFGRX C32S were PCR-amplified with Phusion DNA polymerase using the corresponding p416TEF plasmids as a template 35 and subcloned into the Kpn I and Avr II restriction sites of pET45b in strain XL1-Blue

Toxins (Basel) 15:328 Zhang X, Mei D, Li Y, You M, Wang D, Yao D, Xu Y, Zhai L, Wang Y (2022) Arsenic exposure via drinking water during pregnancy and lactation induces autism-like behaviors in male offspring mice