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After 180 min of anaerobic incubation at 37 C, the entire culture volume was collected into 50 mL conical tubes with pipetting and scraping to capture adherent cells, and the cells were centrifuged at 4000 g for 10 min, yielding pellets with a wet weight of ~100 mg

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To detect LYSET, M6P (scFv M6P), GNPT3HA KI, or the uninduced 10xGCN4, the anti-protein A HRP (PA00-03, Rockland, for LYSET and M6P) or mouse HRP (115-035-046, Jackson labs, for GNPT3HA KI) secondary antibodies were used at 1:10,000 dilution

Antidiabetic, Lipid-Regulating and Antiobesity Effects Many in vivo studies on diabetic animal models already confirmed the beneficial effect of Ole or olive leaf extract rich in Ole in type 2 DM disease