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[PubMed] [Google Scholar] 55.Bhatia S
It provides a buffering system and maintains the physiological pH range and osmotic balance of the culture medium

(3) Ultrasonic extraction in an ice-water bath for 10 min, and leave at -40C overnight

For HPLC measurement, a mixture of acetonitrile and 0.1% formic acid solution (95: 5, v/v) was used as an eluent at a flow rate of 0.3 mL/min
