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Results TNBC cell lines express C/EBP with high LIP to LAP isoform ratios We analysed C/EBP expression in the TNBC (ER, PR, HER2) derived cell lines BT-20, MDA-MB-231, BT-549, HCC1806, MDA-MB-468, Hs 578T and CAL-120, the luminal A derived (ER+, PR+, HER2) cell lines MCF-7, T-47D and ZR-75-1, the HER2-positive derived cell line (ER, PR, HER2+) SK-BR-3, and the luminal B derived (ER+, PR+, HER2+) cell lines BT-474, MDA-MB-361 and ZR-75-30 25 as well as in the untransformed mammary epithelial cell line MCF10A cultured in media supplemented with EGF that is known to stimulate LIP expression through CUGBP1 activation 26

There are several possibilities here: formation of the active HSF-1 homotrimer may be reduced because of increased HSP-70 concentration or by another mechanism, HSF-1 DNA binding may be decreased or shortened, or the ctl-2(ua90)II strain may express less HSF-1 overall