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The hydrophilic residue may cause protein instability or unfavorable substrate accommodation, considering the fact that active sites of CYPs are predominantly hydrophobic in nature 46

To remove some precipitated or denatured substances, the following method is recommended: Wash with 2 column bed volumes of 6M guanidine hydrochloride solution, followed immediately by 5 column bed volumes of PBS, pH 7.4
The 1000 most significant SNPs across all chromosomes were annotated using a custom Python script for the genes within 1 kb distance from these SNPs using the gene context files available from the HapMap project 3
