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Additionally, muscle and liver tissues were dissected and divided into two portions: one was fixed in 4% paraformaldehyde for subsequent histological analysis, and the other was stored at 80 C for further analysis
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perfringens CFUs, isolated muscle was cut into small pieces of 24 mm in TGY medium and dissociated in a gentleMACS C tube (Miltenyi Biotec) using a gentle MACS dissociator (Miltenyi Biotec)
Zaretsky et al., 2022
