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NADPH levels decreased to 69% of their initial concentration, a loss of 0.74 M (Supplementary Figure 1a-f)
Briefly, the cells were incubated for 24 h with NiCl 2 in the concentration range of 0.510 mM
Statistical comparisons between the baseline and corresponding post-test values were analyzed using Bonferronis test for multiple comparisons following ANOVA
The first and rate-limiting step, catalyzed by glutamate-cysteine ligase (GCL, formerly -glutamylcysteine synthetase), forms an unusual -peptide bond between the -carboxyl group of glutamate and the amino group of cysteine
