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Vero cells were detached from the flasks by treatment with trypsin (0.5 mg/ml)/EDTA (0.2 mg/ml) in PBS for 5 min at 37 C
Pharmacologic inhibition of ceramide synthesis (e.g., myriocin, a serine palmitoyltransferase inhibitor) and modulation of lipid droplet metabolism (e.g., T863, a DGAT1 inhibitor) have been reported to attenuate lipotoxicity-induced immune activation in experimental systems (82)
