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The ROS production by NOX is regulated at various levels including the assembly of functional NOX complex, the availability of prosthetic group, flavin adenine dinucleotide, the intracellular concentration of calcium, cell surface receptor signals mediated by G protein-coupled receptors, complement, T cell receptor (TCR), and CD28 (3537, 40, 41)

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In brief, cells were seeded in 24well plates at a density of 1.5 10 4 cells/well and treated with dimethyl sulfoxide (DMSO) or 100 M PFOS for 24 h with or without pretreatment with 10 mM l-Carnitine for 24 h