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IHC, and immunofluorescence staining The femoral heads were fixed in 10% formalin for 48 h, followed by decalcification in 10% ethylenediaminetetraacetic acid (EDTA, 25102-12-9, Sigma-Aldrich) for 4 weeks
Moreover, it is also possible that the internalized GPCR can further augment the acute G protein-mediated response (Irannejad et al., 2013
