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GPX4 enzymatic activity assay Cells were collected in lysis buffer (100 mM Tris pH 7.6, 5 mM EDTA, 1 mM NaN 3 and 0.1% peroxide-free Triton-X100)

10.1161/HYPERTENSIONAHA.109.146282 81

Meanwhile, the MDA content in the testes increased considerably, and reduction in fertility was recorded through a decrease in normal sperm and sperm motility and an increase in abnormal sperm (Sajjadian et al., 2014)

Aspirin use in rheumatoid arthritis patients with increased risk of cardiovascular disease
