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TPO performs two functions in thyroid hormone synthesis (Molina, 2018): TPO oxidizes iodide (I - ) ions to form highly reactive iodine (I + ) that covalently binds to carbon 3 or carbon 5 of tyrosine residues on thyroglobulin to create monoiodinated tyroside (MIT) and diiodinated tyrosine (DIT) residues (see Figure 1)

n = 3 technical replicates representative from 2 independent experiments)