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Abbkine, A23210, United States) for 1 h at 37C, and 40,6-diamidino-2-phenylindole (DAPI) counterstaining was performed for the visualization of cell nuclei

Unlike the results found in previous studies, the effect of high dose N -acetylcysteine on air trapping and airway resistance of chronic obstructive pulmonary disease, a double-blinded, randomized, placebo-controlled trial (HIACE) conducted with 120 patients with stable COPD (58 treated with 600 mg of oral NAC twice a day and 62 with placebo), demonstrated a significant improvement in the function of the small airways, in addition to a reduction in the frequency of exacerbation of the disease [53]

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