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Thus, TNBS promotes two independent mechanisms of injury, GSH depletion and ROS generation, both being required for the manifestation of mucosal injury as GSH limitation renders intestine susceptible to the TNBS-induced ROS overgeneration
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So if either of these denaturing agents are required for your purification, youll want to carefully optimize the concentration that you need, or consider using a different type of affinity tag
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