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Quantitative real-time PCR was performed using Taqman qRT-PCR probes (Applied Biosystems, Foster City, CA) and normalized to tissue -Actin by the C T method using StepOne Software v2.1 (Applied Biosystems, Foster City, CA)

To define which pro-inflammatory/pro-angiogenic cytokines and chemokines could be modulated by ALCAR in PCa, for perspective future clinical trials, we performed cytokine profile analysis and in vitro studies, using four PCa cell lines (PC-3, DU-145, LNCaP, 22Rv1) and one benign prostatic hyperplasia (BPH) cell line

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This process is crucial for many body functions, including maintaining healthy blood flow and supporting cardiovascular health