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The paraffin sections were sequentially subjected to dewaxing and hydration, antigen retrieval, endogenous peroxidase inactivation, blocking, primary antibody incubation, secondary antibody incubation, DAB color development, hematoxylin staining, hematoxylin reverse blue, and dehydration operations

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Acknowledgments The authors would like to thank the Key Technological Project of Jiangxi Province

Nonrecycling (Lysosomal Pathway) Leading to Long-Term Desensitization of 2 Adrenoceptors For most GPCRs, once endocytosed, the inactive receptor, now present in an endosome, is sorted to one of two finite destinations: 1) the degradative pathway by lysosomes or proteasomes or 2) renewal (recycling) pathways either via the trans-Golgi network or directly to the plasma membrane (Bao et al., 2020