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Materials and methods Patients and clinical samples A total of 16 bile juice samples were collected from 6 patients with GB polyps, 6 patients with GB gallstones, and 4 healthy individuals who underwent cholecystectomy at Hanyang University Hospital (Seoul, South Korea)

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Transmission electron microscopy The fixed organoids (described above) were rinsed and incubated overnight in 10% sucrose (in water) at 4 C, and the organoids were dehydrated in graded alcohols (50%, 75%, 96%, 100%), cleared in propylene oxide and embedded in a mixture of Epon 812 and Durcupan (Sigma

doi:10.1007/s11011-014-9493-8 Schousboe A, Scafidi S, Bak LK, Waagepetersen HS, McKenna MC (2014) Glutamate metabolism in the brain focusing on astrocytes
