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The cartridges were washed with 50 l of 80% acetonitrile/0.1% TFA, and the cross-linked peptides were eluted with 25 l of a 3:1 ratio of water:NH 4 OH into a 96-well LoBind plate containing 25 l of 10% formic acid in water to immediately neutralize the pH
The medulla is the innermost layer, which may be absent in fine or light-colored hair
Putnam JB, Ryan MB, Walsh GL, Roth JA
Xia N, Forstermann U, Li H (2014) Resveratrol and endothelial nitric oxide: molecular mechanisms
