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Then, total RNA (2 g) was reversely transcribed into cDNA using Iscript cDNA Synthesis Kit (Bio-Rad, Milan, Italy), following the manufacturers instructions
Practical considerations must be taken into account when preparing and handling peptides in solution
Concerning activating Met kinase domain mutations, Park and Di Renzo and Lee and Aebersold (Park et al., 1999
9 , e2236
