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The neurotoxic effect of cuprizone on oligodendrocytes depends on the presence of pro-inflammatory cytokines secreted by microglia

The primer sequences utilized are provided below:HSV-1-ICP0-F: CTGTCGCCTTACGTGAACAA, HSV-1-ICP0-R: CATCCAGAGGCTGTTCCACT, HSV-1-ICP27-F: CTGGCGGACATTAAGGACAT, HSV-1-ICP27-R:TCAACTCGCAGACACGACTC,HSV-2-F: GATCTACAAGGTCCCGCTCG, HSV-2-R: CACCATCCCGTTCACCTTGA, GAPDH-F: TGAGGCCGGTGCTGAGTATGTCG, GAPDH-R: CCACAGTCTTCTGGGTGGCAGTG Hematoxylin and eosin (H&E) staining Vaginal tissues of mice were fixed in 10% formalin, embedded in paraffin, and stained with hematoxylin and eosin (H&E) according to previously described methods 54

The inclusion of dietary sources in regular diet plays a decisive role in the betaine content of the body but betaine can also be synthesised from choline derivatives

In most cases, muscle spasms and twitches are benign and do not cause any serious harm