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Description
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To precipitate proteins, the samples were incubated for 1 h at 20C, followed by a 15-min centrifugation at 9,000 g at 4C
Given the prevalence of ALP misuse in adolescence and their co-ingestion with opioids, we assessed the short- and long-term consequences of repeated ALP on behavioral sensitivity to morphine as measured by the CPP paradigm
