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Efficacy of a copper peptide complex in reducing hair loss

To prepare crRNAs, 45 M W2034 (T7 promoter) and 45 M W2035-W2141 (separate reaction for each) were combined in annealing buffer (10 mM Tris pH 7.5, 50 mM NaCl, 1 mM EDTA), heated to 95 C for 5 min then cooled to room temperature

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Additionally, mitochondrial calcium ions modulate key enzymes of the tricarboxylic acid cycle, such as pyruvate dehydrogenase, thereby regulating OXPHOS (13)
