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Following incubation under anaerobic conditions at 37 C, the bacterial culture was centrifuged at 4 C for 15 min at 7,000 g and washed twice with sterile phosphate-buffered saline (PBS) (Xia et al., 2022)

Though only a fraction of total CHAC1 is associated with the mitochondria in our cells, we show that mitochondrial CHAC1 supports the matrix cysteine pool and is sufficient to sustain Fe-S protein activity under cystine starvation