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Studies in mice revealed that dietary supplementation with TMAO, choline, or carnitine decreased reverse cholesterol transport (RCT) (Janeiro et al., 2018), a mechanism that counteracts excess cholesterol deposition in peripheral tissues by transporting excess cholesterol to the liver and small intestine (Ross and Glomset, 1973).The major pathway for cholesterol elimination is the metabolic synthesis of bile acids in the liver (Zarate et al., 2016), and it was reported that the size of the total bile acid pool was significantly smaller in mice administered TMAO (Koeth et al., 2013)

Zinc and selenium both play a role in testicular function, spermatozoa and oxygen consumption and may help regulate intratesticular testosterone levels

3, 4, 5-trihydroxybenzoic acid ester or 3, 4, 6- trihydroxybenzoic acid ester have been synthesized

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