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The results of the above-mentioned analyses demonstrated that with the increase in the degree of ferroptosis, the overall resistance of HK-2 cells to oxidation weakened, and the degree of lipid peroxidation increased in the high-concentration CaOx envi-ronment

Additionally, prepare single-use aliquots of reconstituted solution before storage to avoid repeated vial puncture and minimize contamination risk in longer experimental programs

The serum aliquot was obtained and kept frozen at 80C until further use for the plasma enzyme activity assay

For most adults injecting into the abdominal wall with a half-inch needle, 90 degrees is appropriate