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l, Representative western blot images of CTPS1 and H1N76/77D expression in 8 cervical cancer tissues isolated from patients after exposure to radiotherapy

For example, copper ions can lead to GSH depletion through two mechanisms: the direct downregulation via the redox reaction between Cu 2+ and intracellular GSH, and the activation of a Fenton-like reaction by generating Cu + to amplify oxidative stress ( in situ GSH in tumor triggered mCMSNs biodegradation and reduced Cu 2+ into Cu + for enhanced hydroxyl radical production, leading to a powerful inhibition effect on tumors (Liu et al., 2019)

Alpha-actinin (Sigma-Aldrich, mouse monoclonal, 1:2500 dilution) was used as housekeeping protein for normalisation of loading and transfer