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Sterile water contains none

But even with a perfectly stable capsule, the timing still matters

1g of RNA was reverse transcribed into cDNA using a High Capacity cDNA RT Kit (ABI, USA) and real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed using an ABI 7,500 Fast real-time RT-PCR System with a Power SYBR Green Kit (Applied Biosystems, Foster City CA, USA), to detect mRNA levels of CD36, fatty acid synthase (FAS), acetyl-coA carboxylase (ACC)-1, and carnitine palmitoyltransferase (CPT)-1, with -actin as an internal control for mRNA quantification

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