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Cells were washed, colleted with 1 mM EDTA in PBS, pelleted (500 g for 5 min) and re-suspended in isolation medium (320 mM sucrose, 10 mM TrisHCl, 1 mM EDTA, pH 7.4)

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Simultaneously, BPC 157 rats exhibit prominent proliferation of fibroblasts and fibers as well as fibers maturation, providing tenable fibroblast and fibers proliferation with their optimal orientation due to the long axes of the myofibers close to the myotendinous junction with lesser number of fibroblasts, and more amount of reticulin and collagen fibers

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