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Description
Insert the needle into the vial stopper, inject the air (this equalizes pressure and makes drawing easier), then invert the vial and draw the peptide solution to the correct graduation mark on the syringe

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For that reason, the delivery of B12 through injections is a miracle for PA patients, who would otherwise be living a constant nightmare

Two-way ANOVA with repeated measures revealed that no significant treatment (F 1, 9 = 0.112, p = NS), no time effect (F 3, 27 = 1.174, p = NS) and no treatment time effect (F 3, 27 = 1.926, p = NS)
