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After tipping off the supernatant, plates were washed, blotted on paper, and blocked with PBS 1% BSA for 30 min at RT

In accordance with one embodiment, a method for increasing circulating and/or intracellular levels of glutathione includes administering an effective amount of S-acetyl-glutathione (SAG) in the form of delayed-release composition to raise the circulating levels of glutathione to achieve clinically beneficial effects

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