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Am J Physiol Heart Circ Physiol

Methylene blue is used in hematology to differentiate blood cell types and in chemistry as a redox indicator ( a molecule that changes color when introduced to certain changes) because it changes color predictably when exposed to oxidation-reduction reactions, making it useful in chemical analysis, and used as a tool for analyzing cellular metabolism and oxidative stress

I read it can take up to 3 months to see results

For histopathological evaluation, skin specimens were collected on Days 7, 14, and 21 using 4-millimeter punch biopsy instruments from different corners of the left-side wound of each rabbit, immediately after planimetry