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Ex vivo studies demonstrate that berberine and its eight analogs inhibit cancer cell viability in a canine mammary carcinoma cell line and transgenic zebrafish models through apoptosis induction, while zebrafish embryo toxicity experiments are conducted to assess the in vivo safety of berberine [198, 199]
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Furthermore, collagen peptides inhibited expression of matrix metalloproteinases (MMPs) and hyaluronidase, enzymes that degrade collagen and hyaluronic acid, thereby slowing ECM degradation [44]
