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These biomarkers are included in the diagnostic criteria because they can predict both germline and somatic FAS mutations with a post-test probability from 85 to 97% (87% for vitamin B 12 ) and because they are accessible to facilities without the ability to do genetic analysis or functional assays (Teachey et al., 2005

Researchers have suggested that individuals with MTHFR mutations are already more prone to functional deficiencies, and tend to have lower levels of active folate even if their dietary intake is adequate

They are not interchangeable unless specifically indicated by research protocols

Reynolds J.E.F