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Substitution of Met 123 with cysteine in CnrX abolishes Ni(II)-responsive expression and decreases the Co(II)-affinity by ~2000-fold [120]
Ther Adv Urol
Key determinants of GSH synthesis are the availability of the sulfur amino acid precursor, cysteine, and the activity of the rate-limiting enzyme, glutamate cysteine ligase (GCL), which is composed of a catalytic (GCLC) and a modifier (GCLM) subunit
