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Immunoprecipitation was performed with anti-HA magnetic beads (Thermo Scientific), anti-FLAG affinity agarose beads (Sigma-Aldrich) or GFP-Trap (Chromotek)

The reconstitution process involves slowly adding bacteriostatic water to the vial with the lyophilisate, most often along the vessel wall, in order to avoid foaming and degradation of the peptide

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Separate research on TRT and Achilles injury risk found a similarly elevated pattern of tendon injury and subsequent surgery in men on testosterone therapy versus matched controls