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Briefly, the liver was removed from the abdominal cavity and blood vessels visible on the cut surface was cannulated and perfused at a flow rate of 2040 ml/min with perfusion buffer I (8.3 g/L NaCl, 0.5 g/L KCl, 2.4 g/L HEPES and 0.19 g/L EGTA)

Biochim Biophys Acta Mol Basis Dis 1866(5):165721 Udupa S, Nguyen S, Hoang G et al (2019) Upregulation of the glutaminase II pathway contributes to glutamate production upon glutaminase 1 inhibition in pancreatic cancer
