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Cells were washed twice in PBS and lysed in RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% SDS (v/v), 0.5% (w/v) Sodium Deoxycholate, 1% (v/v) Triton X-100 and protease inhibitors (Roche, 05892970001))
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Abbreviations: RCT, randomized controlled trial
Differential changes in CD4+ and CD8+ effector and regulatory T lymphocyte subsets in the testis of rats undergoing autoimmune orchitis
