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Dermatologist tested

Additionally, various molecular techniques, including Western blotting, reverse transcription, quantitative PCR, immunofluorescence, and immunohistochemistry, were utilized to examine the expression levels of angiogenesis-related factors and the Notch/NF-B pathways-related factors

Serum insulin, folic acid, and vitamin B 12 concentrations were assessed through radioimmunoassay, while hs-CRP concentrations were measured through turbidimetric assay

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