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We assess and optimize: Surface charge (cationic, anionic, neutral) Physical stability over time (aggregation, leakage, fusion) Thermal and pH stability (4) Release Profiles Liposomes can be designed for immediate release, delayed release, or stimuli-responsive delivery (e.g., pH-sensitive or temperature-sensitive systems)

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The signal at m/z = 354 with moderate intensity can be assigned to glutathione sulfonic acid GSO 3 H

Lactate dehydrogenase release assay Lactate Dehydrogenase Release Assay Kit (C0016, Beyotime Biotechnology, China) was used to detect the level of LDH in cell culture supernatants