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Respective primary antibodies were purchased from Cell Signaling Technology, except for anti-phospho-SMAD3 (Abcam, Cambridge, UK), anti-Ki67 (MIB-1
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doi:10.14740/jem.v4i3.213 Misra M
As tau becomes hyperphosphorylated, its capacity to support microtubule assembly and stability diminishes, resulting in increased levels of unbound tau that may aggregate into tangles (if not secreted or degraded in the lysosome) [107]
