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Between them, F2 was highly enriched in endosomal protein syntenin and tetraspanin CD81, while there was no endosomal protein enrichment in F3, but the common EVs protein marker Flotillin-1 was detected

To further validate that deficiency of hlh-11 truly affects endogenous lipl-3 and acs-2 expression, we used reverse transcription quantitative polymerase chain reaction (RT-qPCR) to examine lipl-3 and acs-2 transcript levels in wild-type animals and hlh-11 KOs

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