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In 2021, Liang Taos team has proposed that TcdB variants presented highly diversified receptor preferences: TcdB1 binds to two known receptors CSPG4 and FZDs, TcdB2 selectively binds to CSPG4, TcdB3 tends to interact with FZDs, and TcdB4 exerts toxic effects in a CSPG4/FZDs-independent manner (Pan et al., 2021)

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the enzyme stabilization arises that means forming a three-dimensional structure of intermolecular and intramolecular crosslinks that perform as a barrier evading its solubilization in the medium [181], to alterations in the reaction medium production it more resistant, temperature, pH, permitting storage for long periods (up to years), and consenting its recycle easing the parting of the medium

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