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Total protein lysate (residual fraction) was then isolated using RIPA lysis buffer (ThermoFisher) supplied with 1x Complete Protease Inhibitor (Roche)

Besides the function of providing methyl groups for all methylation reactions (including DNA, RNA, and proteins), one-carbon metabolism is also essential for the production of phospholipids and nucleotides during proliferation [10]

American Journal of Human Genetics, 67, 11041109

However, they did not find a significant reduction in total antioxidant capacity [100]
