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Briefly, HepG2 cells were incubated with 200 nM FLAG-tagged macrocycle for 1 h
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under ROS-related stimulation, the phosphorylation of RelA (p65) modulates NF-B activity, influencing the expression of downstream genes involved in inflammation, immunity, and cell growth
In brief, the striatal tissues were homogenized using a Dounce homogenizer in a final 20-fold dilution of ice-cold meta-phosphoric acid solution (MPA, 3%) and EDTA (1 mM)
