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X-linked Charcot-Marie-Tooth disease and progressive-relapsing central demyelinating disease

BDNF serum levels have been determined in many studies to characterize changes in neuropsychiatric disorders such as depression, Alzheimer's disease and others
As research reveals more about the potential causes of depression, more targeted treatments are being developed

A80-229AP, Bethyl Laboratories, Inc.) in PBS/T/M was added for 1 h at room temperature, followed by wash and detection with 1 mg/mL (100 L/well) of p -Nitrophenyl Phosphate Substrate (Sigma-Aldrich) dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich)