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HFF-1 cells maintained in a T-25 cell culture flask (Corning, United States) at 37C in a 5% (v/v) CO 2 atmosphere and harvested at 90% confluence using 0.12% trypsin-EDTA

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Moreover, the multi-copy sod strains were constructed and significantly improved the activity of SOD (2750 U/mg), which is the highest enzyme activity reported in S