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The resulting precipitate was dissolved in an extraction buffer and subjected to ultrasonic disruption using a cell crusher (JY92-IIN, Shanghai Huxi Industrial Co., LTD., China) at 65 W for 30 min

[DOI] [PubMed] [Google Scholar] 213.Barriuso D., Alvarez-Frutos L., Gonzalez-Gutierrez L., Motio O., Kroemer G., Palacios-Ramirez R., Senovilla L

This allowed the research team to determine the animals learned responses by counting how many times each subject crossed the quadrant where the platform was located

For instance, Alox15 knockout mice experience skin issues and increased inflammation [32]
