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PreScission protease-treated proteins were diluted fivefold in buffer A (20 mM Tris-acetate pH 8.0, 1 mM DDM), treated with 1 mM TCEP, and passed through 0.5 mL of pre-equilibrated sulfopropyl(SP)-sepharose fast-flow resin (GE healthcare) twice in a gravity column at room temperature
