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Total retinal protein was determined through the use of Pierce BCA Protein Assay Kit (Thermo Fisher Scientific REF# 23225) with absorbance read using a Cytation 5 (Biotek) plate reader to allow data to be normalized by total protein level in each retinal sample
(2011) Distortions and disconnections: disrupted brain connectivity in autism
doi: 10.1200/JOP.2011.000486 273
Its ability to stimulate endogenous growth hormone production is well established
