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It is always best practice to draw your dose from the vial immediately before use

[DOI] [PMC free article] [PubMed] [Google Scholar] 63.Sweetman E, Kleffmann T, Edgar C, de Lange M, Vallings R, Tate W

Using a well-characterized compound like BPC-157 allows you to isolate the variable you're actually trying to study

Injecting air into the BAC water vial before withdrawing solvent equalizes pressure, making drawing easier and reducing vacuum resistance on the plunger
